Composite

Part:BBa_K607004

Designed by: Olesja Popow   Group: iGEM11_Groningen   (2011-09-14)

PlasB_RFP-LVA

RFP with LVA-tag under transcriptional control of the lasB promoter. In the presence of autoinducermolecule(AI) also called HSL the lasB promoter is induced by LasR. HSL can either be produced by LasI or added to the medium. The RFP has a taq, so it will be degraded faster compared to RFP without LVA-tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 738
    Illegal AgeI site found at 850
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None